[1]秦焕蓉,程芝梅,吴祥锴,等.经导管动脉化疗栓塞和微波消融治疗VX2肝癌诱导低氧诱导因子-1α调节糖酵解与微血管密度相关性分析研究[J].介入放射学杂志,2024,33(04):390-396.
　QIN Huanrong,CHENG Zhimei,WU Xiangkai,et al.Correlation between HIF-1α regulation of glycolysis and microvessel density induced by transcatheter arterial chemoembolization and microwave ablation in experimental rabbits with VX2 hepatocellular carcinoma[J].journal interventional radiology,2024,33(04):390-396.
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经导管动脉化疗栓塞和微波消融治疗VX2肝癌诱导低氧诱导因子-1α调节糖酵解与微血管密度相关性分析研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
33
期数:
2024年04
页码:
390-396
栏目:
实验研究
出版日期:
2024-05-04

文章信息/Info

Title:
Correlation between HIF-1α regulation of glycolysis and microvessel density induced by transcatheter arterial chemoembolization and microwave ablation in experimental rabbits with VX2 hepatocellular carcinoma
作者:
秦焕蓉;  程芝梅;  吴祥锴;  何慧州;  郭韵涵;  王黎洲;  张 帅;  周 石
Author(s):
QIN Huanrong;  CHENG Zhimei;  WU Xiangkai;  HE Huizhou;  GUO Yunhan;  WANG Lizhou;  ZHANG Shuai;  ZHOU Shi.
School of Imaging， Guizhou Medical University， Guiyang， Guizhou Province 550004， China
关键词:
【关键词】 肝肿瘤; 经导管动脉化疗栓塞术; 微波消融; 兔; 模型; 糖酵解
文献标志码:
A
摘要:
【摘要】 目的 探讨经导管动脉化疗栓塞术（TACE）和微波消融（MWA）治疗VX2肝癌诱导的低氧诱导因子（HIF）- 1α糖酵解与微血管密度相关性。方法 选取19只新西兰兔，建立18只VX2肝癌模型。随机分为对照组（建模不做处理，n=6）、TACE组（经肝动脉注射碘化油和吡柔比星乳剂，n=6）、MWA组（经皮穿刺肿瘤后行MWA，n=6）。肿瘤长至1.5～2 cm时行相应手术治疗。术后2周处死实验兔，取肿瘤及瘤周些许肝组织。苏木精- 伊红（HE）染色观察肿瘤细胞形态。免疫组化检测肿瘤HIF- 1α、微血管密度（CD34、CD105标记）、血管内皮细胞生长因子（VEGF）、Ki67表达。蛋白质印迹实验检测糖酵解关键酶己糖激酶（HK）2、丙酮酸激酶（PK）M2、乳酸脱氢酶（LDH）A、葡萄糖转运体（GLUT）1、GLUT4及丙酮酸脱氢酶激酶（PDK）1蛋白表达。结果 18只兔均建模成功，手术成功率为100%。HE染色显示肿瘤细胞排列紊乱。免疫组化检测显示，TACE组、MWA组与对照组相比，HIF- 1α、CD34、CD105、VEGF及Ki67表达升高（均P＜0.05）；蛋白印迹实验分析显示，与对照组相比，TACE组、MWA组术后GLUT1、GLUT4表达升高（均P＜0.05）；PKM2、HK2表达量MWA组升高（均P＜0.05），但TACE组的差异无统计学意义（均P＞0.05）；LDHA、PDK1表达量TACE组升高（均P＜0.05），但MWA组的差异无统计学意义（均P＞0.05）。Pearson相关性分析结果表明，肿瘤内微血管密度与糖酵解代谢呈正相关。结论 TACE和MWA治疗后VX2肝癌模型糖酵解代谢上调，且与肿瘤血管新生正相关。

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备注/Memo

备注/Memo:
（收稿日期：2023- 08- 17）
（本文编辑：谷 珂）
更新日期/Last Update: 2024-05-04