[1]黄 昊,何仕诚,冯国栋,等.骨水泥对兔脊柱VX2肿瘤转移模型的作用研究[J].介入放射学杂志,2015,(06):520-523.
 HUANG Hao,HE Shi- cheng,FENG Guo- dong,et al.The killing effect of bone cement on spinal metastasis of transplanted VX2 carcinoma in experimental rabbit models[J].journal interventional radiology,2015,(06):520-523.
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骨水泥对兔脊柱VX2肿瘤转移模型的作用研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2015年06期
页码:
520-523
栏目:
实验研究
出版日期:
2015-06-25

文章信息/Info

Title:
The killing effect of bone cement on spinal metastasis of transplanted VX2 carcinoma in experimental rabbit models
作者:
黄 昊 何仕诚 冯国栋 杜瑞杰 朱海东 方 文 郭金和 邓 钢
Author(s):
HUANG Hao HE Shi- cheng FENG Guo- dong DU Rui- jie ZHU Hai- dong FANG Wen GUO Jin- he DENG Gang
Department of Radiology, Affiliated Zhongda Hospital, Southeast University, Nanjing, Jiangsu Province 210009, China
关键词:
【关键词】 聚甲基丙烯酸甲酯 VX2肿瘤 脊柱转移 凋亡
文献标志码:
A
摘要:
【摘要】 目的 探讨聚甲基丙烯酸甲酯(PMMA)骨水泥对兔脊柱VX2肿瘤的杀伤作用。方法 成功建立兔VX2脊柱转移肿瘤模型18只,随机分为A、B、C组,每组各6只,在CT导下向VX2肿瘤中心分别注入PMMA 或生理盐水,其中A组注入PMMA 0.3 ml、B组注入PMMA 0.1 ml、C组注入生理盐水0.3 ml。术后24 h处死模型兔,A、B组分别于距离PMMA团块边缘1、5、10、15 mm处不同方向各取4个肿瘤组织块,C组于瘤体中心至表面不同的4个位置各取1块肿瘤组织,采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法检测肿瘤细胞凋亡率。结果 16只模型兔成功注入PMMA骨水泥,A、B组手术成功率均为5/6,C组为6/6,无显著性差异。A组距PMMA边缘1、5、10 mm处肿瘤细胞平均凋亡率分别为(65.75±18.81)%、(50.00±14.24)%、(14.95±8.98)%,与对照组(9.79±5.24)%相比差异均有统计学意义(P<0.05);15 mm处肿瘤细胞平均凋亡率为(10.30±8.13)%,与对照组相比差异无统计学意义。B组距PMMA边缘1、5 mm处肿瘤细胞平均凋亡率分别为(49.20±15.57)%、(17.75±9.28)%,与对照组相比差异均有统计学意义(P<0.05),其余观测点无显著性差异。A、B两组距PMMA边缘1、5、10 mm处肿瘤细胞平均凋亡率比较,差异均有统计学意义(P<0.001)。结论 PMMA可促进肿瘤细胞凋亡,适量增加PMMA注入量可增加肿瘤细胞凋亡范围。

参考文献/References:


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备注/Memo

备注/Memo:
(收稿日期:2014 -11-27)
(本文编辑:边 佶)
更新日期/Last Update: 2015-06-24