[1]朱先海,王伟昱,施长杲,等.温控射频消融治疗VX2肝癌实验研究[J].介入放射学杂志,2016,(01):56-60.
 ZHU Xian- hai,WANG Wei- yu,SHI Chang- gao,et al.Temperature- controlled radiofrequency ablation for the treatment of hepatocellular carcinoma VX2: an experimental study[J].journal interventional radiology,2016,(01):56-60.
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温控射频消融治疗VX2肝癌实验研究 ()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2016年01期
页码:
56-60
栏目:
实验研究
出版日期:
2016-01-25

文章信息/Info

Title:
Temperature- controlled radiofrequency ablation for the treatment of hepatocellular carcinoma VX2: an experimental study
作者:
朱先海 王伟昱 施长杲 秦汉林 吕维富
Author(s):
ZHU Xian- hai WANG Wei- yu SHI Chang- gao QIN Han- lin LV Wei- fu
Department of Cancer Interventional Radiology, Anhui Provincial Tumor Hospital, Affiliated Provincial Hospital of Anhui Medical University, Hefei, Anhui Province 233004, China
关键词:
【关键词】 VX2肝癌 温控模式 射频消融 细胞凋亡 细胞增殖
文献标志码:
A
摘要:
【摘要】 目的 探索不同温度控制条件下射频消融(RFA)治疗兔VX2肝癌后靶区内肿瘤细胞凋亡及增殖变化。方法 采用开腹组织块法构建兔VX2肝癌模型,随机分为对照组(n=6,未行RFA)和实验组A组(n=24,射频针温度50~70℃)、B组(n=24,射频针温度70~90℃)、C组(n=24,射频针温度90~110℃)。实验组分别接受CT引导下不同温控RFA,靶区由消融中心向外周依次分为针道区、消融区和交界区;采用苏木精-伊红(HE)染色观察肿瘤及周围肝组织病理学变化,脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)和增殖细胞核抗原(PCNA)免疫组化法检测各区带肿瘤细胞凋亡及增殖情况。结果 RFA后实验组各组各区带6个时间点(术后即刻,1、3、7、14、21 d)标本肿瘤细胞凋亡指数(AI)和增殖指数(PI)与对照组比较,均有统计学差异(P<0.05);B、C组区带各时间点AI均高于A组(P<0.05),B、C组间无明显差异(P>0.05);术后7、14、21 d时B、C组消融区和交界区PI均低于A组(P<0.05),且术后21 d时C组PI低于B组(P<0.05)。结论 RFA温控在50~110℃能达到基本灭活针道区兔VX2肝癌细胞的作用,选择90~110℃温控更利于彻底控制肿瘤。

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备注/Memo

备注/Memo:
(收稿日期:2015-03-11)
(本文编辑:边 佶)
更新日期/Last Update: 2016-01-22